Aseptic Filling (PFS/Vials) for LAI – PQ Protocol

Document Number: PQ-001

Version: 1.0

Effective Date: YYYY-MM-DD

Reviewed By: [Name]

Approval Date: YYYY-MM-DD

Performance Qualification Protocol for Aseptic Filling of Long-Acting Injectables

Objective: To validate the aseptic filling process for depot suspension products to ensure compliance with regulatory requirements and product specifications.

Scope: This protocol applies to the aseptic filling equipment used for Long-Acting Injectables in the Production area, focusing on the critical parameters impacting product quality.

Responsibilities:

  • Validation Team: Overall execution and documentation of the PQ protocol.
  • Production Personnel: Ensure equipment is operated per SOPs during testing.
  • Quality Assurance: Review and approve all validation documentation.

Prerequisites:

  • Completion of Installation Qualification (IQ) and Operational Qualification (OQ).
  • Availability of all necessary materials and equipment.
  • Training records for personnel involved in the PQ process.

Equipment Description: The Aseptic Filling system is designed for the sterile filling of depot suspension products into pre-filled syringes (PFS) and vials, ensuring a sterile environment and precise fill accuracy.

Test ID Procedure Acceptance Criteria Evidence
PQ-001-01 Verify fill accuracy by measuring filled volume. Volume within ±5% of target fill volume. Calibration records and measurement logs.
PQ-001-02 Assess suspension homogeneity using visual inspection. No phase separation or sedimentation observed. Photographic evidence and inspection logs.
PQ-001-03 Evaluate aseptic controls through environmental monitoring. Microbial counts within acceptable limits. Environmental monitoring reports.
PQ-001-04 Review audit trail for data integrity checks. All entries logged with timestamps and user IDs. Audit trail reports.
See also  Weighing Balance (Platform/Floor) – PQ Protocol

Detailed Test Cases:

  • Test Case 1: Measure the filled volume of 10 syringes and calculate the average fill volume.
  • Test Case 2: Perform a visual inspection of 10 vials for any signs of phase separation.
  • Test Case 3: Conduct environmental monitoring during a filling run; record and analyze results.
  • Test Case 4: Generate and review the audit trail for the filling process to ensure compliance with data integrity standards.

Deviations: Any deviations from the acceptance criteria must be documented and investigated. A deviation report will be generated for all critical failures.

Approvals:

  • Validation Manager: ________________________ Date: __________
  • Quality Assurance: ________________________ Date: __________