HPLC, UV & Analytical Instrument Calibration How to Calibrate HPLC, UV-Vis & Analytical Instruments in Pharma Laboratories Introduction High-Performance Liquid Chromatography (HPLC), UV-Visible spectrophotometers, and other analytical instruments are the backbone of pharmaceutical testing. Their calibration ensures precise, accurate, and reproducible data — a cornerstone of Good Laboratory Practice (GLP) and regulatory compliance. According to FDA Guidance for Industry: Analytical Procedures and Methods Validation, all analytical instruments must be calibrated at defined intervals to verify performance. This article outlines step-by-step protocols for calibration, including regulatory expectations, critical parameters, documentation practices, and risk-based considerations. Regulatory Framework and Standards Calibration must comply…