Media Fill Trials in Aseptic Processing: Protocol Design, Interventions & Acceptance Criteria Executing Media Fill Trials in Aseptic Processing: Protocols, Interventions, and Regulatory Criteria Media fill trials, also known as aseptic process simulations, are a regulatory requirement and critical control measure for demonstrating the sterility assurance of aseptic manufacturing operations. These trials use microbial growth media instead of actual product to mimic the complete aseptic process under worst-case conditions and assess its capability to consistently prevent contamination. This article offers a detailed step-by-step guide to designing, executing, and evaluating media fill trials in compliance with FDA, EMA, and WHO standards….